DNAse and cell clumping

Ralph Bohmer (ralph.bohmer@es.nemc.org)
Tue, 21 Oct 97 9:55:33 EDT

According to the textbooks, DNAseI makes single-strand nicks in the presence
of Mg++ and double-strand breaks in the presence of Mn++ (10mM). However, to
prevent cell clumping, everybody appears to be using DNAse in plain PBS
(probably Ca++ and Mg++ -free).
Mn++ gets never mentioned.
To prevent or undo cell wrapping by DNA, don't we want double-strand breaks ?

Ralph M. Bohmer, New England Medical Center