For some time we have been examining Annexin V- FITC (+ - EDTA) binding.
PI uptake in elutriation purified cryopreserved human monocytes cultured in
low adherence plates and have observed high Annexin V binding at Zero-time
incubation (6o% of the cells) (PI 3-5%). After a short time (less than 4
hours) Annexin V drops to about 15% (PI 3-5%) Similiar values for Annexin
V and PI are observed after 24 hours incubation. Recovery of cells from
the wells is excellent (manuel counting in counting chamber- better than 9o
% at all harvesting times).
We wonder if cell-membrane pertubation (leading to Annexin V binding ) may
be reversible.
In other words may "flip-flop" bee reversible or should it always lead to
apoptosis??
Appreciate any answer/suggestion.
Thanks
Peter Kierulf,MD PhD. Professor I
Clinical Chemistry Dep.
Ulleval University Hospital
N-0407 OSLO, NORWAY
Telephone: +47 22 11 94 86
Telefax: +47 22 11 81 89
_-----------------------------------Mobil telphone: 922.85111
E-mail (RFC Formate): peter.kierulf@ioks.uio.no
E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no
Peter Kierulf,MD PhD. Professor I
Clinical Chemistry Dep.
Ulleval University Hospital
N-0407 OSLO, NORWAY
Telephone: +47 22 11 94 86
Telefax: +47 22 11 81 89
_-----------------------------------Mobil telphone: 922.85111
E-mail (RFC Formate): peter.kierulf@ioks.uio.no
E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no