Cupp, J.E., Leary, J.F., Cernichiari, E., Wood, J.C.S., Doherty,
R.A.:"Rare Event Analysis Methods for Detection of Fetal Red Blood Cells
in Maternal Blood" Cytometry 5: 138-144 (1984).
Corsetti, J.P., Cox, C., Leary, J.F., Cox, M.T., Blumberg, N., Doherty,
R.A.:"A Comparison of Quantitative Acid-Elution Technique and Flow
Cytometry for Detecting Fetomaternal Hemorrhage" Annals of Clin and Lab
Sci 17(3): 197-206 (1987).
Good luck.
James F. Leary, Ph.D.
Professor of Internal Medicine
Chief, Molecular Cytometry Unit
Division of Infectious Diseases
Tel: 409-747-1927; fax: 409-772-6527
On 10/24/97 Wanda wrote:
We are looking at performing quantitation of D positive cells (FMH) by
flow cytometry. I have been trialing Fluro-D (FITC labelled, CSL
product) using the method as recommended by the manufacturer. So far I
have found that my standard curve results (O Neg blood spiked with O Pos
blood) don't always correlate as close as I would expect/like. Is this
due to poor technique or have other operators found similar results. So
far, I have been able to detect low levels of D positive cells with
minimal background. However, I would appreciate any comments from other
users about potential pitfalls, correlation to Kleihauer results, best
method of reporting (% or mls of D cells) and any other general hints.