> Try this:
> While measuring Fl1 against Fl2 add 15 microliters of a 25 micromolar
> solution of calcium ionophore. Look at your cells, within 1-4 minutes
> you'll see the 405 nm emission go up. If you don't see this it means
> you don't have any Indo-1 in your cells and you're measuring autofluorescence.
> Good luck!
> M. Kushnir
> mkushnir@playfair.utoronto.ca
>
Thanks to your informative replied answer.
I'm sorry that I was a bit late to reply it.
I've done the experiment as you suggest,
and it shows that no emission going up.
In my case, I use Fluo-3 instead of Indo-1.
Do you have any idea how to make more Fluo-3
inside the cell?
Thanks in advance.
Best Regards,
Marianti M.