fixing cells for FACS

Court, Rebecca (CourtR@prose.dpi.qld.gov.au)
Fri, 31 Oct 1997 16:04:00 +1000

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Hi everyone,
I have a question with respect to the feasibility of fixing cells for
analysis of oxidative burst and phagocytosis.
I am using Hydroethidine to assess oxidative burst in monocytes and
neutrophils (whole blood) following PMA stimulation. The phagocytosis is
assessed via FITC labelled opsonised zymosan. Is it possible to fix
these cells (if so, what's the best way) to allow for analysis the day
after staining? Will the dye leak from cells after fixation?
Thanks in advance for your help
Rebecca Court

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