I have a user who wants to examine apoptosis in porcine adipocytes and
pre-adipocytes. The scattergrams of the initial preps looked horrible,
so I didn't think we had any intact cells. They insist that the
cells are in good shape microscopically, but there is a 50-fold
variation in cell size. Ok, I changed to log FS, but they don't
look much better. Does anyone have any experience with adipocytes in
flow--specifically tips for preparation? Is anyone looking at apoptosis
in these cells? And finally (since _they_ think the results are
promising)--has anyone has any success with higher concentrations of
Triton X-100 in the tdt assay? The B-M kit suggests 0.1%, and they have
tried up to 0.5%, but they were wondering if they could go higher. I
suspect that we have a soup of debris which will only get soupier, but I
hate to pour water on their fires of enthusiasm.
Thanks for any pearls of wisdom you may have to offer,
Steve
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Steve G. Hilliard steve@habanero.cb.uga.edu
If you're a runner you should be dead.
http://storm.cadcam.iupui.edu/drs/drs.html
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