Washing technique in leukemia analysis

Timothy J Farley (tfarley@computer.net)
Tue, 18 Nov 1997 23:25:20 -0500

I recently was told of a technique involving analysis for antibody staining
of no-washed and then washed cells from a leukemia analysis. The
particular set-up used PB, a Coulter Q-Prep, and a panel from a patient
with leukemia was analyzed. Some specific results (CD19+CD5+) were
confirmed after washing. The conversion of a positive result to negative
after washing was considered significant.

Can anyone familar with the clinical singificance of this technique provide
any background. Thanks!

Tim Farley