Just a small sample of qq that would be useful.
Thanks,
Bob
-----Original Message-----
From: ridder.gm@pg.com [SMTP:ridder.gm@pg.com]
Sent: Saturday, November 15, 1997 1:22 AM
To: Cytometry Mailing List
Subject: Re[2]: Luminex technology
We are working with Luminex and have a very different view of them. The
Coulter machines simply represent a technical interface problem for on-line
analysis; thus they are reluctance to utilize this machine temporarily until
their own machine comes out. However, we use a Coulter Elite ESP to gather the
listmode files and Luminex is modifying their software for batch off-line
analysis; they have been very accommodating and we see no technical limitations.
We visited them in Austin for two days to develop an assay and run our own
samples on their equipment. Running standards for 9 simultaneous analyses
worked as advertised; it was fast and simple. There should be significant cost
savings over ELISA assays, but the exact prices of FlowMetrix kits under
development are not established yet. The sensitivities were not quite as good
as our standard ELISA's, but from other literature on bead analyses and their
previous experience with other assays I believe it could be. We had difficulty
with high backgrounds in running our samples as a washless assay and both our
labs are in the process of tweaking the method. Their new cytometer is very
innovative and may set some new standards in cost and design. In all, we
remain excited by the possibilities, see no fundamental limitations and will
pursue this avenue. But, we're not there yet.
(We have no commercial interest in Luminex Corp)
Gregg M. Ridder
Miami Valley Labs
Procter & Gamble
PO Box 538707
Cincinnati, OH 45253-8707
ridder.gm@pg.com
Phone: (513) 627-2634
FAX: (513) 627-1760
>No, But I can tell you there was hesitancy from the personnel at Luminex
>about doing studies on a Coulter flow cytometer when I offered such at the
>CAC meeting in August. The technology looks exciting, but Luminex remains
>circumspect.
> ----------
>From: Gregor Rothe
>To: cyto-inbox
>Subject: Luminex technology
>Date: Thursday, November 06, 1997 5:44PM
The Austin (TX) based company LUMINEX claims that their microbead
technology allows the simultaneous analysis of 64 parameters in flow
cytometric multi-ligand assays in immunology or molecular biology.
Is their anyone on this list who can comment based on practical
experiences with different applications using their products?
Thanks for any information,
Gregor Rothe
***********************************************************
Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, Germany
Tel. +49 (941) 944-6204
Fax +49 (941) 944-6202
E-mail: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE
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