Confocal Microscopy Instrumentation
Bio-Rad MRC 1024 UV/Vis System

The MRC 1024 installed in the lab consists of the following components:

Lasers:

• UV/Vis: Coherent Innova Enterprise Model 622 - 60 mW output (with remote control) Water Cooled
• Visible: ILT Ar-Kr Model 5470K-00C-2B
  

Microscope:

• Nikon Diaphot 300 (Inverted)
• Objectives:
  • Plan APO 10 x 0.45
  • Fluor Phase 10 x
  • Flour 20 x 0.75
  • Fluor 40 x 1.3 DIC OIL
  • Plan APO 60 x 1.4 DIC OIL
  • Plan APO 100 x 1.4 OIL
  
  
• DIC Condenser and optical components
• EPI - Fluor Attachment with 100 w HG lamp, Powersupply for HBO 100w
  
• Stage:
  • Automated X/Y Stage
• Z-Axis:
  • Computerized Z-Motor model #31002 plus controller
    
• Vibration Table:
  • TMC Model 78-455-12 Size: 8' x 4', drilled 3/16 plate every 1"
  Physiology Components:
  • Heated Stage: DTC-W-120-LC Digital Temperature Controller & Stage Brook Industries made to order (see Picture)
  • Objective Heater: OH-100 Objective Heater Brook Industries made to order

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MRC-1024 Options:

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• 3rd Detector Channel
• Transmission Detector 3 color
• Ethernet Link
• Triple Labelling Filter

Computer System:

• Compaq Prosignia 500 with 80 Meg ram, 21" Monitor

Networking:

• Novell 4.01

Archival Storage/Backup:

• Philips CD Rom Writer
• Corel Optical disk
  

Output/Presentation:

• Printers
• Codonics NP1600 Dye Sublimation Color Printer
• H-P 4M Laser Printer
• Tektronics Color Laser Printer
  

Digital Camera

• Lasergraphics LFR Personal
  

Workstations:

• 2 x Silicon Graphics Workstation (Indigo 2) (see data analysis above)

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Bio-Rad DVC 250 Viewscan
The DVC 250 installed in the lab consists of the following components:

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Lasers:

• Visible: ILT Ar-Kr Model 5470K-00C-2B
  

Microscopes:

• Nikon Diaphot 200 (Inverted) with computerized Z-Motor model #31002 plus controller
• Nikon Labophot (Upright) with computerized Z-Motor model #31002 plus controller

Camera:

• Monochrome: Photonic Sciences "Darkstar"
• Color: Optronics 470T

Computer:

• Compaq Prosignia 500 with 80 Meg ram, 21" Monitor

Image Analysis Software:

• Metamorph/Metafluor, Universal Imaging Corp.

Video Monitor:

• Sony HR Trinitron

Filter Wheel:

• Lambda 10-2, Suter Instrument Company

Video Laserdisc:

• Panasonic TQ-3031F Laser video disc

Physiology Components:

• Heated Stage: DTC-W-120-LC Digital Temperature Controller & Stage Brook Industries made to order (see Picture)
• Objective Heater: OH-100 Objective Heater Brook Industries made to order

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