TITLE:  Scientist, Flow Cytometry (full-time)

REPORTING LOCATION: 1330 Piccard Dr, Rockville, MD 20850

REPORTS TO: Vice President of Scientific Operations  

PRIMARY OBJECTIVES: Support pre-clinical and clinical teams with exceptional immunology research skills.

DUTIES AND RESPONSIBILITIES

·       Perform multi-parameter flow cytometry including panel design (>12 colors), instrument set-up, sample acquisition and data analysis

·       Generate novel methods and/or work with method developers to develop, transfer, and validate appropriate flow cytometry assays

·       Perform ex vivo and in vitro assays for immuno-oncology relevant endpoints such as ELISA and multiplex cytokine assays (e.g. Luminex and MSD)

·       Responsible for quality and efficiency of studies performed within the pre-clinical and clinical laboratory.

·       Lead analysis of pre-clinical and clinical flow cytometry data (using Flowjo, FACS Suite and Diva software) and reporting of results.

·       Responsible for scheduling and effectively planning workload

·       Ensuring compliance with established procedures and environment in a regulated laboratory.

·       Sample preparation (murine or human tissues/cells) for immunostaining and acquisition by flow cytometry

·       Preparation and handling of primary specimens, including peripheral blood, ascites, other liquid biospecimens.

·       Troubleshoot, optimize, and develop methods for unmet experimental needs

·       Interact with other teams and clients as needed.

·       Maintain laboratory records and notebook, organize and compile data, and write weekly reports on results

KNOWLEDGE, SKILLS, AND ABILITIES

·       Proficient in multi-parameter flow cytometry including panel design (>12 colors, instrument set-up, sample acquisition, data analysis)

·       Data analysis experience using Flowjo, Diva software and other FACS related analysis tools

·       Experience with PK/PD, in vitro T-cell functional characterization assays including in vitro cytotoxicity, proliferation, and cytokine/chemokine measurements.

·       Proficiency in the analysis and understanding of immune cells and molecules.

·       Perform ex vivo and in vitro assays for immuno-oncology relevant endpoints (e.g. ELISA and multiplex cytokine assays)

·       Primary immune cell culture (experience with T cells, NK cells, Dendritic cells, Macrophages)

·       Experience in data interpretation and statistical analysis.

·       Ability to coordinate parallel tasks across multiple projects, demonstrating prioritization.

EDUCATION AND EXPERIENCE

·       Ph.D. in immunology, cellular and/or tumor biology or a closely related field

·       Must have experience in flow cytometry, immune cell phenotyping of immune cell subsets (0-2 years Academia or Industry)

·       Experience designing and developing flow cytometry panels for pre-clinical and clinical samples

·       Demonstrated experience with in vitro assay development preferred

·       Demonstrated experience working with pre-clinical models preferred

·       Demonstrated experience in in vivo immunology and oncology tumor model prefer

PHYSICAL DEMANDS AND WORK ENVIRONMENT

·       Must be able to sit and use hands to finger, handle and feel.

·       Must be able to lift and move packages weighing up to 50 pounds.

·       Must be able to sit for long periods of time using a computer in a typical office environment in a multi-level facility

·       Some activities require repetitive motion and reaching, pulling or pushing.

·       In carrying out the duties of the job there will be frequent exposure to fumes and chemical hazards.

·       Duties performed routinely require exposure to blood, body fluid and tissue.

Link:  https://www.paycomonline.net/v4/ats/web.php/jobs/ViewJobDetails?job=15847&clientkey=8E074C8045885157668F3D28842C8B65

This job description may be changed to include new responsibilities and tasks or change existing ones as management deems necessary.