Re: Calcium analysis

Ray Hicks (rh208@cus.cam.ac.uk)
Sun, 11 Feb 1996 14:38:45 +0000 (GMT)

Hi Eric,
You can use FCS Assistant to calculate a ratio for you and output a line
graph of mean ratio versus time, as long as you can get your data across
from the HP to a macintosh. I'll step up my efforts to get it to save
in LYSYS-compatible format if you really want to analyse in LYSYS (but
the facilities are a bit limited, which is why I incorporated the line
graph option). If you've got an IBM it would be worth browsing Eric
Martz's Catalogue of Software, where there are some good "total
packages".

Ray

On Fri, 9 Feb 1996, Eric Miller wrote:

>
> Hi "Flowers(!)"
> Anyone out there doing Calcium analysis on a Facscan? I've been
> having a go using Fluo-3 and Fura red, both separately and in
> combination, with some fairly encouraging results. I use Lysys II on a
> Hewlett- Packard uprated 310 computer, but the analysis side is fairly
> tedious, since I need a 6 minute time course, and the *** computer won't
> do ratioing for me. Has anyone any thoughts about the best way to analyse
> and present these results, given that the analysis has to be done within
> Lysys II? I have tried to get assistance from B-D in the UK without success.
> Please help. If this doesn't work I have been threatened with a
> fluorimeter!
>
>
> Eric P Miller
> Edinburgh Medical Oncology Unit
>
> "I am Pentium of Borg. Division is futile......you will be approximated."
> Star Trek - Generations. Two Captains, one density.
>
>