I would like to know the special tricks to use, if one sorts fixed human
cells for RNA / DNA / Protein extraction afterwards. I think because of
fixation (which has to be done for security reasons), there could be a
problem of RNA degradation etc... Do you add RNAse, DNAase or Protein
inhibitors to the samples ?
I know that this subject was probably already on the subject list, but I
don't have good access to the archives (from Europe it's quite slow).. so I
would still be interested in getting some hints.
thank you very much,
Matthias
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Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (1) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (1) 45 68 86 39 INSTITUT PASTEUR PARIS
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