Admittedly if all one is wanting is is a yea or nay result, as in diagnosing a
Glanzmann's disease or assaying activation, then a proportion of negative RBC's
won't make much of a difference to the final answer.
Consanguinous marriages are the norm here, as is evident from the Thal traits,
so our project will include a large scale assaying of the population for
heterozygous Glanzmann's (we know there are a lot !) where the Fluorescence
means are of prime importance and which ideally should not include any false
negative data.
Also, getting rid of the RBC overlap will allow us to put large numbers of
assays on the XL's carousel and allow the instrument to "autogate" on the pure
PLT populations.
With the conventional non-lysed technique we had to manually gate on the
platelets for each patient.
I shall post this reply to the list, since I am sure that others may have the
same legitimate query as you.
Wal Sharp
SQU
Oman