Re: Selection of channels.

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Strategy seems OK, FITC is the commonest fluorochrome conjugated to
antibodies. I assume when you say CY5 you mean PE/CY5 CD4 , CY5 needs a red
laser.

Simon

Simon Monard
FACS Lab Manager
Aaron Diamond Center for AIDS Research
455 First Avenue
New York
NY 10016

Ph (212) 448 5141
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu