Induction of apoptosis in macrophages/microglia

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I am looking for a positive control for inducing apoptosis in
cultivated macrophages/microglial cells. I did some Annexin/PI stains, however, Colchicin
and growth-factor-withdrawl does not look very promising to me. (It just increases the
number of Annexin/PI-stained "necrotic" cells).
I would highly appreciate any hint.

Joachim v. Zahn
Max-Delbrueck-Centrum
Cellular Neurosciences
Robert-Roessle-Str. 10
13122 Berlin
Germany
jzahn@orion.rz.mdc-berlin.de

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu