PI TOXICITY

Immuno Flow (Immuno_Flow_at_APPLIC1@ccmail.llu.edu)
Thu, 30 Nov 95 12:45:49 PST

My Dearest Friends,

Thanks for lunch! I was hoping that live/dead was an immediate
staining, and most of our current protocols for DNA were 15 min. As
the toxicity goes we see increases in the negative population over
time and I think we need to lower our concentration of PI and add it
just prior to running.

I think I'm in the mood for a four coarse meal today.

'Til my next bet,

Judy

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu