Re: NK Activity

KGERTIS (kgertis.dhl1@mail.unch.unc.edu)
Thu, 30 Nov 95 16:10:00 -0500

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Alice L. Givan: "flowers"
Previous message: Immuno Flow: "PI TOXICITY"

Hi, I don't have any info on Calcein AM, but there is a very nice method
of measuring NK Activity by Flow cytometry using Propidium Iodide and 3,3
dioctadecycloxacarbocyanine (DiO). This method is described in the Journal
of Immunological Methods, 166:45-54, 1993 by Chang, et al. We use a very
similar method in our lab and it is quick, easy and accurate. Hope this
helps. Karla Gertis, UNC Hospitals, Chapel Hill NC

Next message: Alice L. Givan: "flowers"
Previous message: Immuno Flow: "PI TOXICITY"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu