Re: FACScan side scatter problems

Mikael Vestberg (Mikael.Vestberg@inflam.lu.se)
Tue, 19 Dec 95 22:24:04 +0100

>Carol Oxford wrote:
The problem occurs when looking at a histogram of forward vs. side=20
scatter. The sample will be acquiring normally, then the events will=20
"drop" (relative to side scatter) sort of like snowflakes for a second,=20
then begin acquiring normally again.=20

I have also seen this and until now I always thought it was air bubbles=20
coming through. I do not think it is an electrical problem though. I=20
have had the machine plugged into several different phases. We have, I=20
think, 4 different transformers in house and it has been plugged into at=20
least two different ones. I will try to go through them systematically=20
and report back.=20

I have used the same method as Dennis Broud and just gated them out.

Mikael


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu