Re: FACScan side scatter

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We also see the "blips" in SSC. Our DB service engineer and us came
to a conclusion that they are caused by microbubbles (we agree with
Judy). They are NOT caused by electrical surges (we have a good
voltage regulator in line). We do not deal with them during acquisition
(keep acquiring the sample) but we "cut"/gate them out in our analysis
(make a plot of SSC vs TIME (Winlist)). That way you do not have to abort long
acquisition and lose the sample.

Vera

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu