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Fixation of your sample should create cells which in effect are like ball
bearings and thus be discriminated equally regardless of the speed. All
functional properties will be poisoned and thus prevent these cells from
adhering to plastic. However, this does not seem likely and sounds more like a
fluid dynamic problem which might be better explained by Coulter personal.
On our ELITE-ESP some of our sorts are at speeds >2000 cell/s, I have never seen
Mono's disappear even when we have sorted viable cells.
Stephen P. Perfetto
Department of HIV Disease Prevention
Walter Reed Army Institute of Research
1600 East Gude Drive
Rockville, MD. 20850
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Subject: Disappearing monocytes
From: Dave Lundy <dlundy@inforamp.net> at Internet_Gateway
Date: 12/27/95 9:31 AM
I am posting this on behalf of a colleague who is not on the 'net'. He is
running an Elite ESP, equipped with air-cooled lasers, and the 3x 100-micron
flow cell ("Sort-Sense"). The (prepped whole blood) samples run and
accumulate normally, until he increases the sample rate. Once the rate is
above 1000-2000 per second, the monos "disappear" ! Reducing the rate
again brings the monos back into the accumulation. We think this might be
due to the monos binding to the sample tubing, and/or other surfaces. Is
there a way to prevent this?
Regards,
Dave Lundy.
(Coulter Canada Ltd.)
dlundy@inforamp.net
Work: Coulter Canada Ltd., 905 Century Drive, Burlington, Ontario L7L 5J8
TEL: 905-452-9187 FAX: 905-333-3787
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