Disappearing monocytes

Dave Lundy (dlundy@inforamp.net)
Wed, 27 Dec 1995 09:31:36 -0500 (EST)

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I am posting this on behalf of a colleague who is not on the 'net'. He is
running an Elite ESP, equipped with air-cooled lasers, and the 3x 100-micron
flow cell ("Sort-Sense"). The (prepped whole blood) samples run and
accumulate normally, until he increases the sample rate. Once the rate is
above 1000-2000 per second, the monos "disappear" ! Reducing the rate
again brings the monos back into the accumulation. We think this might be
due to the monos binding to the sample tubing, and/or other surfaces. Is
there a way to prevent this?
Regards,

Dave Lundy.
(Coulter Canada Ltd.)
dlundy@inforamp.net
Work: Coulter Canada Ltd., 905 Century Drive, Burlington, Ontario L7L 5J8
TEL: 905-452-9187 FAX: 905-333-3787

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu