Re: FACScan side scatter problems
Dennis Broud 301-594-6259 FAX 301-443-9292 (BROUDD@fdacd.bitnet)
Thu, 21 Dec 1995 10:28:42 -0500 (EST)
I would occasionally see them with the LysisII setup as well. I suspect that
my problems are more along the line of "air bubbles" rather than crystals, as
all my staining and dilution buffers, as well as tissue culture media are
filtered through 0.2 micron filters before use. It is however somewhat
strange or at least serendipitous that these "air bubbles" have the same
forward scatter range as human lymphocytes. The BD service man seems to think
that it looks more like an intermitant communications problem between the
cytometer and the computer.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
