Debra Klich's Q.
Bill James (bjames@oncor.com)
11 Jan 1996 15:23:03 -0500
Subject: Time: 3:05 PM
OFFICE MEMO Debra Klich's Q. Date: 1/11/96
The question:
"Debra Klich
I am looking for a protocol for=20
>the reliable quantification of bulk DNA/RNA from tissue. I've tried a=20
>standard digestion and extraction followed by spectrophotometric=20
>readings at 260nm. I feel that I am loosing a lot during the=20
>extraction. Does anyone have any infomation that may help?!?!"
To Debra:
Oncor sells a product called "Ex-Wax" which is used for extracting nucleic acids
from paraffin-embedded tissue (disclaimer: I work at Oncor R & D). If you want
product info call (800) 776-6267. Molecular Probes sells great DNA binding dyes
such as Pico-Green for DNA fluorometric quantitation (I've no relationship).
Bill James
bjames@oncor.com
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
