TUNEL
Dan Smith (DJS@ALLP.COM)
Thu, 11 Jan 96 09:02:00 PST
I am forwarding a request from a coworker regarding getting a GOOD positive
control to work in our TUNEL/apoptosis assay. Which nuclease have you
used (company, ph#, etc) with the best results.
Thanks again!
Dan Smith
San Diego
e-mail: djs@allp.com (or through the users group)
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We have been unable to get the positive control to work in her TUNEL
protocols. The control involves treating the permeabilized cells with a
nuclease to nick the DNA and render it TUNEL positive. It must be a problem
with the enzyme itself since we are registering what we interpret as TUNEL
positive cells in the treated samples. Could you submit a question to the
internet group concerning what nuclease and vendor might help us solve this
problem. We could just start ordering different ones from different places
but this may give us a more reasonable approach. Long live the NET!
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
