Re: Indo-1 standard beads

Dave Coder (dave@nucleus.immunol.washington.edu)
Tue, 16 Jan 96 08:03:31 -0800

Jeff Wang at Spherotech 1-800-368-0822 (or 708-680-8922, 708-680-8927 FAX)
(near Chicago) produced some several years ago when he was with Pandex. He
may have some still around. Keep in mind, however, that the emission spectrum
of the dye on a bead may vary with respect to the emission spectra of indo-1
within different cells. But for your stated purposes, they could work quite
well.

Dave Coder
dcoder@u.washington.edu

Begin forwarded message:

Date: Fri, 12 Jan 1996 16:05:25 -0600
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From: Julie Auger <jauger@flowcity.bsd.uchicago.edu>
Subject: Indo-1 standard beads
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Does anyone know, off the top of their head, if there are any fluorescent
beads available that mimic Indo-1? I know there are beads that are uv
excitable and emit blue, but it would be nice to have beads that excite in
the uv and have the same emission spectra as free indo-1 or ca++bound
indo-1. They would be useful to me for instrumentation alignment/setup as
well as for teaching purposes.

Thanks,

Julie
********************************************************************************
Julie A. Auger voice: 312-702-9212 lab
Director, Flow Cytometry Facility 312-702-9261 office
University of Chicago fax: 312-702-3701
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