GFP and FACS-Gal/FDG
Hank Pletcher (pletcher@mail.med.upenn.edu)
Wed, 24 Jan 1996 18:14:52 -0500
I received a call from an investigator who would like to do two reporter
gene constructs simultaneously. He is considering the new Green
Fluorescent Protein mutant as well as the FACS-Gal/FDG assay. The problem
of course is that both of these fluoresce green.
We were wondering if there are any red substrates for lacZ that have been
used in flow with decent sensitivity.
Any suggestions on alternative methods would also be appreciated. They
would prefer not to use an antibody mediated detection of their protein
product.
Thanks in advance for any advice.
Hank
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Hank Pletcher (pletcher@mail.med.upenn.edu)
University of Pennsylvania Cancer Center
Flow Cytometry and Cell Sorting Facility
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