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Eric P Miller
Edinburgh Medical Oncology Unit
"I am Pentium of Borg. Division is futile......you will be approximated."
Star Trek - Generations. Two Captains, one density.
On Thu, 25 Jan 1996, Gary Durack wrote:
> This is a post from a colleague ...
>
> Cytometry Experts,
>
> We are doing flowcytometric analysis of nuclear DNA content and ploidity
> determination of paraffin embedded archival pathological lymphoma cases in
> dogs.We dewax the 30um sections by two changes of xyleneand dehydrate
> with sequential ethanol concentrations and distilled water.Finally we
> digest the tissue by pepsin and stain by standard PI staining method.
> We get nice diploid and aneuploid peaks,however the fluorescence of the
> deparaffinized samples is lower than in the fresh material.
>
> Questions:Is this the expected pattern of paraffin embedded tissue?
> If yes:what is the explaination?
> If not:how can we increase PI uptake in archival lymphoid tissues?
>
> farkas@ux1.cso.uiuc.edu
>
> Amnon Farkas
> Univeristy of Illinois
> School of Veterinary Medicine
>
> Gary Durack
> University of Illinois Biotechnology Center
> Flow Cytometry Facility
>
> Voice (217) 244-0559
> FAX (217) 244-0466
>
>
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