murine spleen/ B-cell subsets

David Gebhard (gebhard@aecom.yu.edu)
Thu, 1 Feb 1996 16:30:20 -0500

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Is there a specific marker or combination of markers that will
discriminate B-cells in each of the three murine splenic compartments
(follicular/germinal center, marginal zone, PALS(peri arteriole lymphatic
sheath)? My user is interested in any suggestions for identifying and
"sorting/separating" cells from these regions, as well as
separating/identifying plasma from memory cells arising in germinal centers
during primary immune response. Is there a good resource we can avail
ourselves of?

Thanks
Dave Gebhard
Director FACS/ Oligonucelotide Synthesis Facilities
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, New York 10461
718-430-2724/ 3573

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu