lacZ gene expression/3 color FACS

"facs1::isdellf"@rmslab.rockefeller.edu
Wed, 31 Jan 1996 10:33:07 EST

Dear Colleagues,

Does anyone have experience using Molecular Probes LacZ gene expression Kits.

At the moment we are considering using ImaGene Green, and CD3/PE and
HLA-DR/Per-CP for gene product detection in T and Dendrtic cells.

Does anyone have any practical exprience regarding which acyl chain length we
should use - we really don't want to permeabilize the cells. Also, which would
you recommend the DetectaGene kit or the ImaGene kit.

We will be accquiring data on a BD FACScan but later want to sort for gene
expression using a FACStar Plus equipped with a 488nm argon ion laser - we also
have secondary HeNe and HeCd lasers.

Any tips, suggestions would be greatly appreciated.

Thank you

Frank Isdell/Davorka Bilicic
The Rockefeller University\
New York

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