re: macrophage analysis

Robin Barclay (100066.300@compuserve.com)
29 Jan 96 20:19:29 EST

>>Anyone have a trick for either removing macrophages from plastic, or better
still, preventing them from sticking in the first place? <<
Lidocaine (lignocaine) and some of its relatives will make them jump off plastic
- there is a literature on this subject from the '70's - tried it again recently
- still works. Ca/Mg depletion helps.
Robin Barclay
SNBTS: Edinburgh


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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu