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We have developed a model system for isolating large quantities
of almost pure eosinophils from the lungs of mice infected with the eggs
of the canine roundworm, Toxocara canis. These cells were analyzed by
flow cytometry for the presence of Fc receptors for IgG, A, M, and
especially IgE. We compared these cells to B lymphocytes in the spleen
of the same animals. In answer to the question posed, we used light
scatter both SS and FS to gate on the relevant population. We published
this work in
Jones RE, Finkelman FD, Hester RB, and Kayes SG: Toxocara
canis: Failure to find IgE receptors (Fcepsilon R) on eosinophils from
infected mice suggsesst that murine eosinophils do not kill helminth
larvae by an IgE-dependent mechanism. Exp. Parasitol. 78: 64-75.
We verified that the cells in our gates were in fact eosinophils
by cytosmear staining. We did by the way (BTW) find the 2.4G2 receptor
for aggregated IgG on mouse eos and this was the only Fc receptor we
found. Mouse eos are not like their human counterparts when it comes to
surface fuzzies.
Steve Kayes
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/\ /\//\/######/ /\/#######\ ! Stephen G. Kayes, Ph.D. !
/\/ /\//\/ /\/ /\/ ! Structural & Cellular Biology !
/\/ /\//\/ /\/ /\/ ! University of South Alabama !
/\/ /\/ \/#######/ /\/########/ ! Mobile AL 36688 !
/\/ /\/ /\/ /\/ /\/ =================================
\/------\/ /\-----/\/ /\/ /\/ Office: (334) 460-6768
/#######/ \/####/\/ \/ \/ FAX: (334) 460-6771
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On Mon, 29 Jan 1996, Howard Shapiro wrote:
>
> >would one be able to identify mouse eos. in the same way as described for
> >humans?
> By polarized scatter, definitely, according to Leon Terstappen; by
> fluorescence, probably.
> --Howard
>
>
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