Re: macrophage analysis
Scott I. Simon (ssimon@bcm.tmc.edu)
Fri, 2 Feb 1996 11:18:54 -0600 (CST)
Dear Richaed;
One method for harvesting Mono's is a Dynal bead magnetic particle
separation. Using the CD14 beads it is possible to sterily separate
monocytes from monocytic cells. The yield is high and clean. Hope this helps;
scott simon
baylor college of medicine
On Fri, 26 Jan 1996, RICHARD L. DARLEY wrote:
> Anyone have a trick for either removing macrophages from plastic, or
> better still, preventing them from sticking in the first place.
>
> Surely there must be a better way than scraping them off, which is a
> pain for the small-scale cultures we are running - also I suspect
> many of them get damaged in the process.
>
>
> ################################################################
>
> From: Dr Richard Darley
> LRF Preleukaemia Unit
> Department of Haematology
> UWCM
> Cardiff, CF4 4XN
> U.K.
> tel (+44) 12 22 74 45 24
> Fax (+44) 12 22 74 45 23
> e-mail: darley@cardiff.ac.uk
>
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