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From: monahanb@navmed.nci.nih.gov Tue
Could you direct me to a person I could ask a question
regarding cell cycle analysis. I have completed a drug exposure
experiment with the MCF-7 cell line and completed a cell cycle
analysis
using PI staining with the BD CellFit analysis software on our FACSort
machine. The data quality was good,however I am unsure as to selecing
the best model to fit to the data. The data demonstrates a large
number of cells ( >50%) are in the g2/M arrest phase. The CellFit
softtware though doesn't seem to adequately model cell populations
that have a large G2/M component. What is the most generally accepted
way to treat an analysis of a cell culture whose cells are largely in
G2/M phase using the BD CellFit software ?
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Please post all responses to the web not to my address.
J.Paul Robinson, Purdue University Cytometry Labs
Professor of Immunopharmacology
robinson@flowcyt.cyto.purdue.edu PH:317-494 6449 FAX:317-494 0517
web http://www.cyto.purdue.edu
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