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I routinely use 2 750 instruments for 3 and 4 color applications. There
are a couple of solutions for your compensation woes. A hardware approach
is to acquire a "subtraction module" from an old EPICS V system. This
will plug right into the lower rack of your 750 and give you another
compensation channel. This has the benefit of allowing you to correct
the 3rd color (Pe-Cy5) using a PE signal already corrected for the FITC
overlap. Alternatively Cytomation has done a pretty good job with adding
compensation to their system. You can compensate the 3rd channel there
and use it for sorting. We have Ciceros on our systems.
gary
Gary Durack
University of Illinois at Urbana-Champaign
Biotechnology Center Flow Cytometry Facility
217 244 0559
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