Re: Dead cells pre-fixation
Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
08 Oct 96 10:39:07 EDT
Hello Flowers,
With regard to Denis Snider's question about excluding cells that were dead
prior to fixation, there is an interesting paper in Cytometry 19:243-255
(1995) by O'Brien and Bolton --- which uses an anti-tubulin-FITC stain prior to
fixation to mark permeable (i.e. dead) cells.
Alice Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, NH 03756 USA
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
