Re: Moab to CD40L

Enoc Hollemweguer (enoc@pharmingen.com)
11 Oct 1996 10:49:27 -0700

RE>>Moab to CD40L
CD40L is expressed on stimulated T cells. Using a combination of PMA and Ca
Ionophore, the cells are incubated for 5 to 6 hrs to induce the expression of the
antigen. Longer incubation times results in loss of CD40L expression.
Detailed protocol is published on:
Kroczek, R.A. et al. 1994. Immunological Reviews 138: 39-59.

--------------------------------------
Date: 10/10/96 7:01 PM
To: cyto-inbox
From: Ronald L. Rabin, M.D.

>Hi
> I'm looking for a commercial source of monoclonal/s to CD40 ligand.
>If not available I'm considering the possibility of biotinylating
>CD40 and then using a streptavidin label. Has anyone had any
>experience of this?
>Any comments would be most appreciated
>Thanks
>Jane
>jhughes@ich.uct.ac.za
>Fax 021 689 1287
>Tel 021 658 5313

Pharmingen has a PE labelled anti-CD40L. I don't know how good it is,
since the cells I have stained so far have been completely negativel

Ronald L. Rabin, M.D.
Clinical Associate
Cytokine Biology Unit
LCI/NIAID NIH
phone: (301) 492-4910
fax: (301) 496-7383
e-mail: rr84g@nih.gov

------------------ RFC822 Header Follows ------------------
>From flowcyt.cyto.purdue.edu!owner-cyto-sendout Thu Oct 10 18:22:23 1996 remote
from donews
Received: from flowcyt.cyto.purdue.edu by donews.cts.com with smtp
(Smail3.1.29.1 #5) id m0vBWIw-0000IwC; Thu, 10 Oct 96 18:22 PDT
Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO)
for cyto-sendout id RAA24185; Thu, 10 Oct 1996 17:47:47 -0500
Received: from alw.nih.gov by flowcyt.cyto.purdue.edu via SMTP (940816.SGI.8.6.9/930416.SGI.AUTO)
for <cytometry@flowcyt.cyto.purdue.edu> id KAA20514; Thu, 10 Oct 1996 10:15:35
-0500
Received: from odie.niaid.nih.gov by alw.nih.gov (5.61/1.35(alw-2.4))
id AA11114; Thu, 10 Oct 96 11:14:31 -0400
Received: from [128.231.240.183] (db0183.niaid.nih.gov) by odie.niaid.nih.gov
(5.67b/mg-1.53)
id AA04295; Thu, 10 Oct 1996 11:14:30 -0400
X-Sender: rrabin@pop.niaid.nih.gov (Unverified)
Message-Id: <v02120c00ae821a4e63e2@[128.231.240.183]>
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Date: Thu, 10 Oct 1996 11:14:33 +0800
To: cyto-inbox
From: rr84g@nih.gov (Ronald L. Rabin, M.D.)
Subject: Re: Moab to CD40L


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu