Increasing cytoplasmic fluorescence - final
Walter Sharp (102675.320@CompuServe.COM)
09 Nov 96 20:41:26 EST
Dear All,
A bit embarassing this - it turns out to be the TO from the Retic runs
that is lighting up permeablised cells !
We assumed that our cleaning method at the end of the Retic runs was effective
enough since the tubing is supposed to be relatively immune to dye absorption.
Now I understand the saying "Assuming just makes an ASS of U and ME".
A couple of Methanol and DW tubes cleans it up.
Gold stars to those who made the right deduction and apologies to the rest of
the list for asking the question BEFORE I'd definitively ruled everything else
out.
You can't win 'em all !
Wal Sharp
SQU
Oman.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu