Figure 1: Typical red vs. green fluorescence histogram of Staphylococcus aureus treated with 70% ETOH and stained with BacLight Kit. Control for region 4 (dead).

Figure 2: Histogram of Staphylococcus aureus incubated at room temperature and stained with BacLight Kit. Control for region 2 (live).

Figure 3: Bivariate contour plot for Staphylococcus aureus heat incubated at 60 degrees Celsius and stained with the BacLight Kit. Region 1 is a mixture of viable and non-viable bacteria.

Figure 4: Typical pattern for Staphylococcus aureus treated with Vancomycin (0.5 ug/ml). Bacteria in regions 3 and 4 are non-viable. Region 1 is a mixed population with both viable and non-viable cells.

Figure 5:Contour plot of Staphylococcus aureus treated with Vancomycin (2.0 ug/ml). Regions 3 and 4 are non-viable. Region 1 contained a mixture of viable and non-viable bacteria. Upon overnight incubation the viable (Vancomycin resistant) population predominated, overgrew the culture, and stained as a single live population.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu