Luciferase labeled probe

vannt (ntvan@audumla.mdacc.tmc.edu)
Mon, 3 Feb 97 15:07:03 CST

I have an urgent need to know the excitation and emission wavelength for
luciferase in neutral pH. I want to know if bound to orotein in cells,
luciferease undergoes a bathmochromic effect(red shift). What is the best
filter and excitation wavelength and emission wavelength for flowcytometric
determination of luciferase.
Thank you in advance


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu