Re: MESF-PerCP, APC

Keith Bahjat (Kbahjat@nwu.edu)
Fri, 7 Feb 1997 09:37:29 -0600

>Dear colleagues,
>
>we are using FCSC beads for calculation of the MESF values of FITC
>and PE. I am wondering if there is a source of beads for the calculation of
>MESF of PerCP or APC. Any help is wellcome.
>Thank you
>
>Attila Tarnok
>
>
>
>***************************************************************
> Dr. Attila Tarnok phone:**49-3418652430
> Clinics for Pediatric Cardiology fax: **49-3418651405
> Heart Center Leipzig GmbH
> University Hospital, Russenstr.19
> D-04289 Leipzig, Germany
> Internet:tarnok@server3.medizin.uni-leipzig.de
>***************************************************************

We use Rainbow Calibration beads from Spherotech, which have eight peaks
that flouresce in all three PMTs simultaneously and include
standardizations for FITC (MEFL), PE (MEPE), and Cy5 (MECY). They are also
very inexpensive. Here's their info.

Spherotech, Inc.
1840 Industrial Drive, Suite 270
Libertyville, Illinois 60048-9817
phone: 847-680-8922
fax: 847-680-8927
Product #: RCP-30-5A (Sphero Rainbow Calibration Particles)

good luck

Keith Bahjat
Kbahjat@nwu.edu


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu