We use Rainbow Calibration beads from Spherotech, which have eight peaks
that flouresce in all three PMTs simultaneously and include
standardizations for FITC (MEFL), PE (MEPE), and Cy5 (MECY). They are also
very inexpensive. Here's their info.
Spherotech, Inc.
1840 Industrial Drive, Suite 270
Libertyville, Illinois 60048-9817
phone: 847-680-8922
fax: 847-680-8927
Product #: RCP-30-5A (Sphero Rainbow Calibration Particles)
good luck
Keith Bahjat
Kbahjat@nwu.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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