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Can anybody help me in the matter of using TRAP-6 to activate platelets?
How much TRAP do you use to maximally activate platelets? I mean how many
micro mol per how many platelets? The papers I have read mention only the
ammount of TRAP (usually 100uM).
I had some problems with activating plts with TRAP - seems like I used too
many plts, or too little TRAP, but maybe the problem is to be sought
elswhere...
The second question to the illuminati is how to determine the proper
compensation for the WB 2 color platelet activation analysis?
Many thanx,
Janek
______________________________________________
Jan L. Brozek e-mail: mmbrozek@cyf-kr.edu.pl
http://www.cyf-kr.edu.pl/~mmbrozek
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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