 |
 |
 |
 |
 |
The increase in P-selectin will saturate with 20 uM/l TRAP-6
and after 10 min using 1:10 PBS diluted whole blood at RT.
If that does not work, what about organic solvents or azide
in your assay?
>
> The second question to the illuminati is how to determine the proper
> compensation for the WB 2 color platelet activation analysis?
>
Set a live-gate in forward versus side scatter on platelets and
compensate the fluorescence of either FITC or PE stained platelets to
that of unstained controls. Spectrally separated fluorochromes
e.g. PE and PerCP or FITC and PE/Cy5 are preferable due
to the high difference of staining intensity between activation
dependent (e.g. CD62P) and constitutive antigens (e.g. CD41)
which are useful for gating.
Gregor Rothe
*************************************************************************
Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, Germany
Tel. +49 (941) 944-6204
Fax +49 (941) 944-6202
Internet: Gregor.Rothe@klinik.uni-regensburg.de
*************************************************************************
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web