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We too are experiencing connection problems with our FACSCalibur. However,
our problems only started AFTER we got the second laser installed. The
timing between when we turn on the cytometer to when we switch on the
computer is well within the magical 1-5 min window. However, we often
(almost every other time) have connection problems. Shutting down &
restarting the computer doesn't seem to help - only a total shutdown of
cytometer & computer works...most of the time (sometimes you have to do this
many times).
Occasionally everything connects properly but we cannot see events. The
magical solution to this problem seems to be to tighten the Bal Seal, however
sometimes this doesn't work. We've tried all the 'solutions' offered in the
troubleshooting section & we were just wondering if anyone else has
experienced this problem.
Seeing the various messages on this topic makes us feel better as we had the
impression that there was nothing wrong with the machines (that no one else
experiences this problem) & that it was just us and our inexperience. So
thank you fellow FLOWers!
We have also noticed that when we switched to the PPC from the Quadra, we now
get a message that says that there is an error in the connection & that the
cytometer cannot be found when we only want to analyse (ie we do not turn the
cytometer on). If you click OK, then you can just analyse, but before we
upgraded we never had this problem!
Thanks in advance for your help
Maribel Leong
Dept Med, University of Melbourne
Australia
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web