 |
 |
 |
 |
 |
I have had trouble getting the cytoplasm stripped from the nuclei using
lepidopteran (Manduca sexta) hemocytes.
I have used fresh, unfixed cells, paraformaldehyde-fixed cells, and
ethanol-fixed cells and stained with a modified Vindelov's PI. Increasing the
detergent (Nonidet P-40) concentration from 0.1% to 0.2% or 0.3% didn't seem
to help. I still see intact cells in the microscope.
Any suggestions? Thank you very much.
C. A. Hallberg
Department of Entomology
Purdue University
West Lafayette, IN 47907
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web