CD45 Negative PBL
Mark Rehse (markrehse@cellpro.com)
Thu, 6 Mar 1997 09:42:09 -0800
Dear Flow Community,
We use CD45 FITC (Immunotech clone J33) in our two color
immunophenotyping panels to identify the WBC component in apheresis
and buffy coat and eliminate RBC's, debris and dim staining platelets
from subsequent enumeration of T and B cells, stem cells, NK cells,
monocytes and granulocytes using PE-conjugated antibodies. This
method is superior to traditional light scatter gating, however, there is a
population of CD45- cells which are equivalent in size and granularity to
T and B cells which we have not yet been able to identify in our panels.
Does anyone have experience with this population? Is it a problem with
this clone?
Thanks in advance.
MarkRehse@CellPro.com
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
