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> From: Mark Rehse <markrehse@CellPro.com>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: CD45 Negative PBL
> Date: Thursday, March 06, 1997 12:42 PM
>
>
>
> Dear Flow Community,
> We use CD45 FITC (Immunotech clone J33) in our two color
> immunophenotyping panels to identify the WBC component in apheresis
> and buffy coat and eliminate RBC's, debris and dim staining platelets
> from subsequent enumeration of T and B cells, stem cells, NK cells,
> monocytes and granulocytes using PE-conjugated antibodies. This
> method is superior to traditional light scatter gating, however, there is
a
> population of CD45- cells which are equivalent in size and granularity to
> T and B cells which we have not yet been able to identify in our panels.
> Does anyone have experience with this population? Is it a problem with
> this clone?
> Thanks in advance.
> MarkRehse@CellPro.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web