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We used a method published in: Bennet, R. et al. The production and
characterization of murine monoclonal antibodies to a DNA receptor on human
leukocytes, J. Immunology 140: 2937-2942, 1988.
I don't believe that polymerized acrylamide is toxic, but in this procedure, we
eluted the protein and removed the bits of acrylamide by centrifuging.
Best wishes,
Tony Bakke
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
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