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We are trying to label CHO cells in suspension with both anti-FAK (Focal
Adhesion Kinase) and anti-VIN (vinculin). We get great labelling with
the cells grown on coversips for confocal, but nothing when they are in
suspension. Has anyone done either of these on cells in suspension, or
do the receptors disappear when the adherent cells are removed from their
substrate???
Thanks in advance.
Deb Berglund
Montana State University
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web