Re: PI staining of PBLs
Terry Hoy (Hoy@cardiff.ac.uk)
Thu, 12 Dec 1996 09:45:19 GMT
Mike Ormerod wrote :
>I have run into a probelm staining peripheral blood cells
>with propidium iodide for cell cycle analysis. .........
>We consitently obtain two peaks in the DNA histogram. A
>lower peak from the lymphocytes and a peak from the
>granulocytes with about 30% more fluorescence.
>
Mike,
We noticed this several years ago looking at separated populations of
lymphocytes, monocytes and granulocytes from peripheral blood
(unpublished) and also with myeloid and erythroid cells separated from
bone marrow. (Hodgetts et al J. Clin. Pathol. 1988; 41;1120-1124).
The differences we noticed were all less than 10% - not as high as
you are reporting.
We thought this resulted from different degrees of chromatin
condensation in the various lineages and opted for measuring
separated populations each with its own 'lineage' control.
All that work was by the Vindelov method.
Terry.
Dr. Terry Hoy
Principal Research Officer
Department of Haematology Telephone 44 1222 743458
University of Wales College of Medicine FAX 44 1222 744655
Heath Park E-mail hoy@cardiff.ac.uk
CARDIFF CF4 4XN http://www.cf.ac.uk/uwcm/hg/hoy/index.html
U.K.
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