Re: A clean machine -Reply

Betsy R. Robertson (brrob@ims.alaska.edu)
Mon, 10 Feb 1997 08:21:44 -0900 (AKST)

Small beads staying in the instrument can be a problem. Triton X-100 at
0.1% (the same concentration used to permeabilize cells) in water will
remove most of them.

Betsy Robertson
=========================================================
Betsy R. Robertson (907)474-7709 - Voice
Institute of Marine Science 474-7204 - FAX
University of Alaska Fairbanks
Fairbanks, Alaska 99775-1080 brrob@ims.alaska.edu
=========================================================


On Fri, 7 Feb 1997, Antony Bakke wrote:

>
> Nona Sheila R. Agawin asked:
> " is it always very hard to clean the flow cytometer after passing samples with
> fluorescent beads ?"
>
> I have found that small beads (< 4 micron) can stay in the instrument for a long
> time appearing in multiple samples after the beads were run. Running ethanol
> after the beads seems to help push them through more quickly.
>
> Tony Bakke
>
>
>


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu